All the study Zenith Labs Pure Greens subjects were between 1 and 6 years old, and had taken no antibiotics or probiotics for the preceding six months. The information on diet came from parents. Not surprisingly the Italian subjects consumed more calories per day, 1512 vs. 996 among two to six year olds.The study authors provided material that supports the idea that human's digestive organisms changed quite a bit once agriculture took hold and the society was no longer nomadic... a scant 10,000 years ago.
Once the food supply changed, the number and types of bacteria changed too, perhaps bringing more diseases. Once antibiotics, vaccines and better hygiene took hold, some scientists believe that more changes in the organisms took place - coincidentally at the same time medicine was seeing an increased number of allergic and autoimmune diseases.Researchers wonder if the bacteria that populate the small Western stomachs might predispose these subjects to obesity or allergies later in life. DNA analysis found that Italian children had more bacteria linked with obesity with fewer fatty acids that can cut down on inflammation. The populations of bacteria were less diverse in the Western diet eating subjects.